Development of a cell sorting platform based on magnetic levitation principle için kapak resmi
Development of a cell sorting platform based on magnetic levitation principle
Başlık:
Development of a cell sorting platform based on magnetic levitation principle
Yazar:
Yılmaz, Esra, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xi, 73 leaves: charts;+ 1 computer laser optical disc.
Özet:
Circulating Tumor Cells (CTCs) play a vital role in cancer diagnosis, prognosis and personalized medicine. However, CTCs are extremely rare in blood (i.e., down to 1- 100 CTC per 1 mL human blood) and hard to isolate because of the heterogeneity of CTCs in biomarker expression. The current CTC separation techniques use numerous differences between cells such as size, electric charges, density and expression of cell surface markers. However, these techniques have many limitations in terms of manual sample preparation steps, inconsistent results caused by low specificity and efficiency, and increased cost. Hence, there is no standard method for isolating CTCs yet. With this study, it was aimed to fill the gap in CTC isolation by proposing a new method based on magnetic levitation principle, which has recently been demonstrated as a highly acceptable method for biological characterization of cells and monitoring of their cellular events. Short while ago, magnetic levitation technology has been used to measure cell densities at single-cell level. By using this technology, unique differences in levitation height and so in density have been identified between cancer cells and blood cells. In this study, we have been developed a new label-free microfluidic cell sorter that is based on the principles of magnetic levitation. After successfully completing this master thesis, this device can be used for rapid, low cost and label-free in-vitro diagnosis of cancer by sorting CTCs from whole blood in a high-throughput manner. The sorted cells might further be collected for downstream analysis for personalized and precision medicine.
Konu Başlığı:
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology: Biotechnology.

İzmir Institute of Technology: Biotechnology--Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.
Ayırtma: Copies: