Preparation of hydroxyapatite/silk protein thin film implant surfaces, investigation of their microstructural properties and model protein interactions için kapak resmi
Preparation of hydroxyapatite/silk protein thin film implant surfaces, investigation of their microstructural properties and model protein interactions
Başlık:
Preparation of hydroxyapatite/silk protein thin film implant surfaces, investigation of their microstructural properties and model protein interactions
Yazar:
Özcan, Selçuk.
Yazar Ek Girişi:
Yayın Bilgileri:
[s.l.]: [s.n.], 2009.
Fiziksel Tanımlama:
xx, 267 leaves.: ill. + 1 computer laser optical disc.
Özet:
Biocompatible hydroxyapatite (HAp) coatings of load bearing metallic in vivo hard tissue implants act as local scaffolds for enhanced osteoconduction, providing fast bone apposition and cementless fixation. In this study, in an attempt to exploit the potential of hydroxyapatite as a carrier of bone morphogenetic proteins for post operative accelerated healing, and implant durability, the tailored microstructural properties, and protein adsorption capabilities of thin film hydroxyapatite implant surfaces were investigated.A novel particulate sol method was used to fabricate HAp thin films on bioinert glass, and Ti6Al4V substrates by dip and spin coating. The microstructural characterization of the thin films was carried out by SEM/EDX, AFM, XRD, and FTIR, and their surface roughness, Vickers hardness and adhesion strength were determined. The effects of silk fibroin and sericin thin film layers on the HAp film microstructure, and model protein (bovine serum albumin, BSA) adsorption behavior (by the size exclusion HPLC method) were investigated. The minimum threshold solid content of the suspensions was determined as 15% by weight for a continuous HAp film structure. The silk sericin and fibroin intermediate layers drastically improved homogeneity of the HAp layer. The BSA adsorption of the glass/sericin/commercial-HAp film was 2.6 ug/cm2, more than twice of the glass/commercial-HAp, and glass/sericin/dry-milled-HAp films, evidencing the effectiveness of surface micro/nano topographical structure as well as chemical structure. The XRD patterns of spin coated commercial-HAp films on Ti6Al4V pointed out to a particular crystal orientation which increased the positive degree of cooperativity between HAp and proteins during adsorption or deposition.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis(Doctoral)--İzmir Institute of Technology: Chemical Engineering.

İzmir Institute of Technology: Chemical Engineering--Thesis (Doctoral).
Elektronik Erişim:
Access to Electronic Version.
Ayırtma: Copies: