Development and characterization of electrospun polymer scaffolds for 3D cell culture model to mimic human intestine system için kapak resmi
Development and characterization of electrospun polymer scaffolds for 3D cell culture model to mimic human intestine system
Başlık:
Development and characterization of electrospun polymer scaffolds for 3D cell culture model to mimic human intestine system
Yazar:
Firik, Tuğba, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
vii, 74 leaves: charts;+ 1 computer laser optical disc.
Özet:
Intestinal tissue engineering is a subfield of tissue engineering, which utilizes biomaterials to mimic the extracellular matrix (ECM) for tissue regeneration, repairment, simulating intestine function in 3D models. It combines scaffolds, which are obtained from natural, semisynthetic or synthetic polymer by using different fabrication methods, with various intestinal cells. In this thesis, polycarbonate (PC), polycaprolactone (PCL) and Polyvinylidene fluoride (PVDF), which are synthetic polymers, were used for fabrication of scaffolds by electrospinning to obtain an alternative material against commercial Transwell supports. Thus, electrospun PC, PCL and PVDF scaffold containing a 12-well Transwell insert was designed by sandwiching between two PMMA circles. To observe cellular behavior on scaffolds Caco-2 cells, which are most used human colorectal cancer cells in the intestinal tissue engineering system, were cultured on electrospun PC, PCL and PVDF scaffolds. According to cell viability analysis, toxic effect was not observed at the end of 21 days culturing period. Additionally, DAPI/Actin and collagen staining was performed for 21-days and results proved that intra/intercellular components and proteins were expressed successfully. As known in literature, Caco-2 cells make a tight junction that is formed by paracellular interactions after 3-4 weeks culturing. To analyze tight junction integrity, phenol red transport and transepithelial resistance were measured. The permeability of phenol red for PCL scaffold exhibited a significant decrease from 50% to 12% after 21 days on the contrary of PC and PVDF. In parallel, TEER measurements supported the phenol red transport assay because in PCL results showed that on the 7th day TEER was measured 468 􀈍􀀑􀁆􀁐2 in average and 21st day measurement was calculated approximately 1133 􀈍􀀑􀁆m2.
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology:Bioengineering.

İzmir Institute of Technology: Bioengineering --Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.
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