Investigating the function of CX26I30N and D50Y mutations in soquamous cell carcinoma cell line SCC-25 için kapak resmi
Investigating the function of CX26I30N and D50Y mutations in soquamous cell carcinoma cell line SCC-25
Başlık:
Investigating the function of CX26I30N and D50Y mutations in soquamous cell carcinoma cell line SCC-25
Yazar:
Yavuz, Büşra, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
viii, 38 leaves: charts;+ 1 computer laser optical disc.
Özet:
Gap junctions are responsible for cell homeostasis and provide cell-cell and cell- ECM communications in multicellular organisms. Gap junction hemichannels consist of connexin (Cx) proteins which functions are crucial for human physiology. Moreover, Cx mutations are associated with various genetic diseases. For example, more than 10 missense mutations in the Cx26 gene cause keratitis-ichthyosis-deafness (KID) syndrome. Among these, Cx26-I30N and Cx26-D50Y cause to form abnormal hemichannels and allow excessive calcium influx into the cell. Furthermore, KID patients have higher squamous cell carcinoma (SCC) incidence and SCC is seen at an earlier age in these patients. Immunohistochemistry stainings have demonstrated aberrant E-cadherin and 𝛽-catenin spread in KID patients’ samples. In this study, we aimed to effects of Cx26-I30N and Cx26-D50Y mutations associated with KID syndrome in human epithelial SCC cell line SCC-25. For this purpose, stable MSCV, Cx26-WT, Cx26-I30N and Cx26-D50Y cell lines were generated and RTqPCR, Western blotting, immunostaining and MTT assays were done for expression level, localization and viability analysis. For these 4 different cell conditions, a significant increase was detected at Cx26 mRNA levels, but not at protein levels. No difference was found in Cx43 level, which is abundant in epithelial tissue, and was localized in the perinuclear area like Cx26. Additionally, E-cadherin protein levels increased and their localization changed in parallel with the mutations from the perinuclear area to the plasma membrane. Moreover, significant decreases in viability were observed in cells grown in high extracellular Ca2+ medium compared to control in contrast to cells grown in Ca2+-free medium.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.

İzmir Institute of Technology: Molecular Biology and Genetics--Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.
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