Heterokaryon Technique for Analysis of Cell Type-specific Localization
Başlık:
Heterokaryon Technique for Analysis of Cell Type-specific Localization
Yazar:
Gammal, Roseann
Yazar Ek Girişi:
Yayın Bilgileri:
Cambridge, MA MyJoVE Corp 2016
Fiziksel Tanımlama:
online resource (571 seconds)
Seri:
Biology
Genel Not:
Title from resource description page
Özet:
A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is described. This heterokaryon approach uses fluorescently-labeled fusion proteins to image protein localizations after cell fusion. The protocol is amenable to steady-state localizations or more dynamic determinations based on live cell imaging.
Okuma Düzeyi:
For undergraduate, graduate, and professional students
Konu Başlığı:
Elektronik Erişim:
https://www.jove.com/t/2488