Molecular and genetic investigation of aging: The role of mitochondrial metabolism genes on life span determination için kapak resmi
Molecular and genetic investigation of aging: The role of mitochondrial metabolism genes on life span determination
Başlık:
Molecular and genetic investigation of aging: The role of mitochondrial metabolism genes on life span determination
Yazar:
Khandaker, Asfaqul Muid, author.
Fiziksel Tanımlama:
ix, 42 leaves: color illustraltions.+ 1 computer laser optical disc.
Özet:
Molecular mechanism of aging and longevity is still a complex phenomenon. In the course of time, an organism or tissue or a post mitotic cell ages, becomes weak, starts losing energy and ultimately falls in death; that implies that mitochondria has a central role of in the aging process. So in this study it is subjected whether manipulations to mitochondrial metabolism genes can extend life span in yeast. 144 strains derived from the yeast (S.cerevisiae) open reading frame (ORF) deletion collection were screened to identify single deleted mitochondrial genes that increase life span. This has resulted in the isolation of three long-lived mutants’ Δppa2 (28% extended), Δdss1(20% extended) and Δafg3 (40% extended) that are chosen for the current study. These long lived cells comprised relatively less amount of mtDNA at the young stage with effective proliferation rate while mtDNA content was highly decreased in old compared to wild type. Relatively less amount of ATP and absence of endogenous reactive oxygen species (ROS) level was observed both in long lived young and old cells. Long lived cell’s mitochondria was viewed as aggregated. In addition, the elevation of the mitochondrial membrane potential (ΔΨmito ) was found to predominate the relative degree of longevity. All long lived cells comprised similar pleiotropic mitochondrial phenotype and whole genome microarray published the sets of genes that were commonly upregulated and downregulated. The induction of peroxisomal glyoxylate cycle along with TCA cycle is suggested upon CIT2 higher expression. Thus this investigation reveals the regulatory properties of these genes through the remodeling of mitochondrial morphology and function.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Doctoral)--İzmir Institute of Technology: Molecular Biology and Genetics.

İzmir Institute of Technology: Molecular Biology and Genetics.--Thesis (Doctoral).
Elektronik Erişim:
Access to Electronic Versiyon.
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