Comparison of connnexin32 expression and function between MCF10A normal breast and MDA-MB-231 breast cancer cell lines için kapak resmi
Comparison of connnexin32 expression and function between MCF10A normal breast and MDA-MB-231 breast cancer cell lines
Başlık:
Comparison of connnexin32 expression and function between MCF10A normal breast and MDA-MB-231 breast cancer cell lines
Yazar:
Adak, Aslı, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
ix, 40 leaves: color illustraltions.+ 1 computer laser optical disc.
Özet:
Breast cancer is one of the most prominent cancer-related deaths among females. Among many molecules, connexins have role in breast cancer. Gap junctions, formed from connexins (Cx), facilitate intercellular communication between adjacent cells. Different connexins were expressed during different stages of breast cancer. Cx32 was found both in normal pre-menopausal and tumor breast tissue samples. In lymph node metastases, elevated Cx32 level was observed compared to primary cancer. However, the role of Cx32 in breast cancer is not known but its elevation in lymph node metastasis may indicate its diverse functions in breast cancer. To verify this, in MCF10A and MDA-MB-231 cell lines, Cx32 was overexpressed. The protein localization was compared with immunostaining. In MDAMB- 231 cells, Cx32 localized in nucleus and cytoplasm, although in MDA-MB-231 Cx32-EGFP cells, Cx32 localized mostly in the cytoplasm. In MCF10A cells, Cx32 localized in nucleus, whereas Cx32 formed gap junctional plaques between MCF10A Cx32-EGFP cells. By Cx32 overexpression, gap junction coupling increased in MCF10A cells significantly, although it did not change in MDA-MB-231 cells. In both cells, hemichannel activity was not altered with Cx32 overexpression. The effects of Cx32 overexpression on cell viability demonstrated a significant decrease in MCF10A cells and an increasing trend in MDA-MB-231 cells. Furthermore, the percentage of G1 phase decreased, G2 and S phases increased in MDA-MB-231. However, Cx32 overexpression did not alter cell cycle profile of MCF10A significantly. Determination of the differential role of Cx32 in different stages of breast cancer may help to understand its diagnostic and/or therapeutic potential.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology: Molecular Biology and Genetics.

İzmir Institute of Technology: Molecular Biology and Genetics--Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.
Ayırtma: Copies: