Education is a significant part of human life as people spend important part of their lives in learning spaces. Various studies indicate that the quality of physical environment in learning spaces has a crucial impact on students' mood, motivation, performance, productivity, health, and well-being. There are many different physical conditions in an environment and among these factors lighting is one of the most important ones as it is a visible condition. Although there is a wealth of research on the effects of lighting conditions on students’ learning, well-being, visual comfort, and performance in regular classrooms, there is a dearth of research about lighting conditions’ effects in architecture design studios. Moreover, while research on the effects of lighting conditions on students’ performance and well-being is abundant research that focuses on motivation levels of the students is scarce. In this regard, the aim of this study is to investigate how lighting conditions affect the visual comfort, satisfaction, and motivation levels of architecture students. Field measurements and surveys were utilized in three architecture studios. Findings demonstrated that lighting conditions have an important impact on students’ visual comfort, satisfaction, and motivation levels. Lack of daylight illuminance, glare, improper artificial lighting, and insufficient lighting were all found to contribute to inappropriate lighting conditions. Lighting conditions have also been found to affect seating preference of users. Seating arrangement and distance from windows affected the perception of users regarding lighting conditions. Based upon the results, some suggestions that would help resolve problems caused by inappropriate lighting were also offered.