Front Cover -- Magnetic Cell Separation -- Copyright Page -- Contents -- Preface -- Chapter 1: Magnetic susceptibility -- 1.1. Mass and volume magnetic susceptibilities -- 1.2. Molar magnetic susceptibility -- 1.3. Magnetic susceptibilities in CGS and SI systems of units -- 1.4. Bulk magnetic susceptibility of a mixture -- 1.5. Volume magnetic susceptibility and Bohr magneton -- 1.6. Multiphase magnetic suspensions -- 1.7. Magnetic phase separation -- 1.7.1. Stationary fluid -- 1.7.2. Laminar flow -- References -- Chapter 2: Magnetic formulary -- 2.1. Langevin theory of paramagnetism -- 2.2. Paramagnetic substances: lanthanide solutions -- 2.3. Paramagnetic substances: hemoglobin and its derivatives -- 2.4. Superparamagnetic particles and ferrofluids -- 2.5. Ferromagnetism and magnetic properties of iron -- 2.6. Diamagnetism -- 2.7. Magnetic field vectors -- 2.8. Magnetic field magnitude -- 2.9. Magnetic field sources -- 2.10. Field gradients -- 2.11. Magnetic field lines -- 2.12. Magnetic field in matter -- References -- Chapter 3: Maxwell stress and magnetic force -- 3.1. Introduction -- 3.2. Magnetic force density and the body force -- 3.3. Magnetic force acting on a small, magnetically susceptible particle (diamagnetic and paramagnetic materials) -- 3.4. Magnetic force on a small particle acting as a permanent magnetic dipole (ferromagnetic materials) -- 3.5. Potential energy of an elementary magnetic dipole -- 3.6. Magnetic field-induced particle motion -- 3.7. Magnetic pathlines -- 3.8. Cell deposition contour surfaces -- References -- Chapter 4: Basic magnetic field configurations -- 4.1. Introduction -- 4.2. Infinite cylinder -- 4.3. Sphere and a stack of spheres -- 4.4. Interpolar gap -- 4.5. Isodynamic field -- 4.6. Quadrupole field -- 4.7. Other magnet configurations -- References -- Chapter 5: Magnetophoresis.

5.1. Magnetophoretic mobility -- 5.1.1. Cross-section for magnetic capture -- References -- Chapter 6: Synthesis and characterization of nano- and micron-sized iron oxide and iron particles for biomedic -- 6.1. Introduction -- 6.2. Maghemite nanoparticles of narrow size distribution -- 6.2.1. Synthesis and characterization -- 6.2.2. Surface modification -- 6.2.3. Biomedical applications -- 6.2.4. MRI contrast agents -- 6.2.5. X-ray contrast agents -- 6.3. Air-stable iron nanocrystalline particles -- 6.3.1. Conclusions -- 6.4. Solid and hollow maghemite/polystyrene and silica/maghemite/polystyrene micron-sized composite particles of narro -- 6.4.1. Synthesis and characterization of solid and hollow microspheres -- 6.4.2. Synthesis of immunomagnetic microspheres for specific removal of ASA and sperm cells -- 6.5. Magnetic/nonmagnetic polystyrene/poly(methyl methacrylate) hemispherical composite micron-sized particles -- Summary -- References -- Chapter 7: The biocompatibility and toxicity of magnetic particles -- 7.1. Introduction -- 7.2. Definition of toxicity and biocompatibility -- 7.2.1. Biomaterials classifications -- 7.3. Particle characteristics that influence toxicity -- 7.3.1. Particle size -- 7.3.2. Particle surface properties -- 7.3.3. Promotion of oxidative processes -- 7.3.4. Leachables -- 7.4. Testing for particle toxicity and biocompatibility -- 7.4.1. Toxicity testing in vitro, in vivo and in silico -- 7.4.2. In vitro biocompatibility and toxicity testing procedures -- 7.4.3. In vivo biocompatibility and toxicity testing procedures -- 7.5. Immunogenicity of biological targeting reagents -- 7.5.1. Immune response system elements -- 7.5.2. Types of hypersensitivity reactions -- 7.6. Legal standards for toxicity testing -- 7.6.1. Efforts to develop risk-based safety evaluations for nanomaterials in the United States.

7.6.2. Efforts to develop risk-based safety evaluations for nanomaterials in Europe -- 7.6.3. Efforts to develop risk-based safety evaluations for nanomaterials in Japan -- 7.7. Conclusions -- References -- Chapter 8: Analytical magnetic techniques in biology -- 8.1. Introduction -- 8.2. Measurements of magnetic susceptibility -- 8.3. Measurements of magnetophoretic mobility: cell-tracking velocimetry (CTV) -- 8.4. Simultaneous measurements of sedimentation rate and magnetophoretic mobility -- 8.5. Magnetophoretic mobility and antibody binding capacity (ABC) -- 8.6. Antibody-magnetic nanoparticle binding to cells -- 8.7. Effect of antibody labeling concentration (antibody titration) on magnetophoretic mobility -- References -- Chapter 9: Preparative applications of magnetic separation in biology and medicine -- 9.1. Introduction -- 9.1.1. Types of methodologies used in magnetic cell separation -- 9.1.2. Types of entities targeted for magnetic cell separation -- 9.1.3. Measures of performance -- 9.2. Examples of positive selection -- 9.3. Depletion of undesirable cells -- 9.4. Enrichment of rare cells by depletion of normal cells -- References -- Chapter 10: Commercial magnetic cell separation instruments and reagents -- 10.1. Invitrogen DYNAL Magnetic Particle Concentrator -- 10.2. R&D Systems Immunicon MagCellect TM Ferrofluid -- 10.3. Miltenyi Biotec GmbH -- 10.4. StemCell Technologies, Inc. -- 10.4.1. Positive cell selection -- 10.4.2. Negative selection -- 10.5. Immunicon Corporation -- 10.6. BD Biosciences -- References -- Chapter 11: Worked examples of cell sample preparation and magnetic separation procedures -- 11.1. Dynalreg T Cell Negative Isolation Kit -- 11.1.1. Principle of isolation -- 11.1.2. Description of materials -- 11.1.3. Protocols -- 11.2. Dynal CD34 Progenitor Cell Selection System -- 11.2.1. Principle of isolation.

11.2.2. Description of materials -- 11.2.3. Protocols -- 11.3. Miltenyi Biotec MACSreg T Cell Isolation Kit II (human) -- 11.3.1. Principle of MACSreg separation -- 11.3.2. Background and product applications -- 11.3.3. Reagent and instrument requirements -- 11.3.4. Protocols -- 11.4. Miltenyi Biotec Direct CD34 Progenitor Cell Isolation Kit -- 11.4.1. Research applications of CD34 progenitor cells -- 11.4.2. Isolation strategy -- 11.4.3. Components -- 11.4.4. Equipment required -- 11.4.5. Protocols -- 11.5. MACS Progenitor Cell Kit performance evaluation -- 11.5.1. Materials and methods -- 11.5.2. Results -- 11.6. Post-separation analyses in other magnetic separation systems -- 11.6.1. Progenitor cell purity analysis by flow cytometry -- 11.6.2. Progenitor cell yield (recovery) and nonprogenitor cells retention frequency analysis by cell counter -- 11.6.3. Progenitor cell morphology analysis by cytospin and microscope -- 11.6.4. Progenitor cell function analysis by cell colony forming unit (CFU) assay -- 11.7. Companies and brand names mentioned in Chapters 11 and 12 -- References -- Chapter 12: New challenges and opportunities -- 12.1. Introduction -- 12.2. Magnetophoresis and magnetic capture of malaria-infected erythrocytes -- 12.3. Magnetic flow cell sorting -- 12.3.1. Continuous cell sorting in the quadrupole field -- 12.3.2. Predicted sorter output based on cell magnetophoresis -- 12.3.3. Calculation of cell recovery and purity of QMS separation -- 12.3.4. Determination of the flow rate parameters for high resolving power and throughput separation -- 12.3.5. The cell model system -- 12.3.6. Analysis of cell fluorescence intensity distribution by FCM -- 12.3.7. Analysis of the cell magnetophoretic mobility distribution by CTV -- 12.3.8. Comparison of magnetic and fluorescent cell fractions -- 12.3.9. Flow rate optimization.

12.3.10. Sorted cell assay by both FCM and CTV -- 12.3.11. Experimental verification of the predicted decrease of CD34+ cell recovery with the increasing Q(a)/Q -- 12.3.12. CD34- cell nonspecific crossover decreases with the increasing Q(a)/Q -- 12.3.13. CD34+ cell purity increases with the increasing Q(a)/Q -- 12.3.14. Mean CD34+ cell fluorescence intensity increases with the increasing Q(a)/Q -- 12.4. Magnetic field-flow fractionation -- 12.4.1. Field-flow fractionation -- 12.4.2. Separation in parallel plate channels -- 12.4.3. Separation in cylindrical and annular channels -- 12.4.4. Earlier implementations of magnetic FFF -- 12.4.5. Interaction of particles with fields -- 12.4.6. Force on particles in a magnetic field -- 12.4.7. Theory for retention in quadrupole MgFFF -- References -- Appendix A: Nomenclature, abbreviations, units, and conversion factors -- A.1. Nomenclature -- A.2. Abbreviations -- A.3. Selected units and conversion factors. -- Appendix B: Vector notation -- References -- Appendix C: Magnetic body force and the Maxwell stresses -- C.1. Magnetic body force and the Maxwell stresses -- C.2. Maxwell stress on a sphere -- C.3. Magnetic force on a particle in the limit of small particle size -- C.4. Elastic body, discrete surface magnetization -- References -- Appendix D: Volume magnetic susceptibilities of selected substances -- References -- Index.