Investigation of molecular effect of phenolic acids on methicillin-resistant and methicillin-susceptible Staphylococcus aureus in comparison to their phenolic acid resistant mutants için kapak resmi
Investigation of molecular effect of phenolic acids on methicillin-resistant and methicillin-susceptible Staphylococcus aureus in comparison to their phenolic acid resistant mutants
Başlık:
Investigation of molecular effect of phenolic acids on methicillin-resistant and methicillin-susceptible Staphylococcus aureus in comparison to their phenolic acid resistant mutants
Yazar:
Keman, Deniz, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xviii, 173 leaves: charts;+ 1 computer laser optical disc.
Özet:
Staphylococcus aureus is a Gram-positive bacterium whose acquisition through an open wound results in various infections. Methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) strains are responsible for diseases ranging from soft tissue infections to fatal pneumonia which cannot be treated due to multiple drug-resistances of these strains. This situation increases the importance of searching for alternative antimicrobials worldwide. Having all these in mind, the capacity of phenolic acids cannot be denied to be used against pathogenic bacteria. Phenolic acids produced as plant secondary metabolites show antibacterial effects besides many beneficial properties for human health. The aim of this study was to investigate the antibacterial action mechanisms of vanillic acid and 2-hydroxycinnamic acid on MRSA and MSSA. To achieve this, firstly the antibacterial effects of phenolic acids on both bacteria were investigated by determination of minimum inhibitory concentrations. Then, the resistance development ability of bacteria against phenolic acids was tested by continuous exposure to subinhibitory concentrations. Finally, the action mechanisms of phenolic acids on bacteria were elucidated using two different proteomic approaches. According to the results, bacteria were not able to develop resistance against phenolic acids. Proteomic studies displayed alterations in the protein profiles of phenolic acid treated bacteria and provided potential targets in the battle with pathogenic bacteria. By showing the inability of MRSA and MSSA to develop resistance to phenolic acids and the important proteomic alterations that are induced by phenolic acid treatment, this study highlights the significance of phenolic acids to be used against antibiotic-resistant bacteria.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Doctoral)--İzmir Institute of Technology: Molecular Biology and Genetics.

İzmir Institute of Technology: Molecular Biology and Genetics.--Thesis (Doctoral).
Elektronik Erişim:
Access to Electronic Versiyon.
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