Detection of the metastatic potential of breast canser cell lines to spesific target tissues için kapak resmi
Detection of the metastatic potential of breast canser cell lines to spesific target tissues
Başlık:
Detection of the metastatic potential of breast canser cell lines to spesific target tissues
Yazar:
Fıratlıgil Yıldırır, Burcu, author.
Fiziksel Tanımlama:
x, 99 leaves: color illustrations, charts;+ 1 computer laser optical disc.
Özet:
Breast cancer is one of the most frequently diagnosed cancer types and the second leading cause of cancer-associated deaths in women. Breast cancer begins as a local disease which can then metastasize to distant sites specifically to bone, lung and liver. The increasing rate of the metastasis-related deaths asserts the need to develop in vitro diagnostic strategies representing in vivo properties better. In this study, two different lab-on-a-chip (LOC) platforms, IC- and EX-chips, were used to detect the invasion and extravasation potentials, respectively, of breast cancer cells to 3D in vitro generated bone, lung, liver and breast microenvironments. The metastatic MDAMB231, but not non-metastatic MCF7 breast cancer cells showed higher invasion and extravasation potentials towards lung and liver microenvironments than breast microenvironment. Lung-specific but not bone-specific metastatic subclonal cells invaded significantly towards lung microenvironment. On the other hand, an intensive invasion was observed in bone-specific but not lung-specific metastatic subclonal cells towards bone microenvironment demonstrating different in vivo metastatic behaviors of breast cancer cells. Overall, the tissue-specific invasion and extravasation capacities of breast cancer cells were demonstrated with IC- and EX-chips where the physiologically more relevant bone, lung, liver and breast homing target sites were generated by a specific emphasis on ECM components, stromal cells and secreted factors. This study is important in providing a basis for the development of diagnostic tools and precision therapeutics for breast cancer metastasis.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Doctoral)--İzmir Institute of Technology:Molecular Biology and Genetics.

İzmir Institute of Technology:Molecular Biology and Genetics. --Thesis (Doctoral)
Elektronik Erişim:
Access to Electronic Versiyon.
Ayırtma: Copies: