Chitosan-plasmid DNA nanoparticles: Cytotoxic and cytostatic effects on human cell lines için kapak resmi
Chitosan-plasmid DNA nanoparticles: Cytotoxic and cytostatic effects on human cell lines
Başlık:
Chitosan-plasmid DNA nanoparticles: Cytotoxic and cytostatic effects on human cell lines
Yazar:
Bor, Gizem, author.
Yazar Ek Girişi:
Fiziksel Tanımlama:
xii, 74 leaves: color illustraltions.+ 1 computer laser optical disc.
Özet:
Although chitosan nanoparticles (CNs) became a promising tool for several biological and medical applications owing to their inherent biocompatibility and biodegrability, studies regarding their effects on cytotoxicity and cytostatic properties still remain insufficient. Therefore, in the present study, we decided to perform comprehensive analysis of the interactions between CNs – pKindling-Red-Mito (pDNA) and different cell line models derived from blood system and human solid tissues cancers. The resulting CNs-pDNA was investigated with regard to their physical-chemical properties, cellular uptake and transfection efficiency, cytotoxic and cytostatic properties. The nanoparticles showed high encapsulation efficiency and physical stability even after 2 days for various formulations. Moreover, high gene expression levels were observed already 96 h after transfection. CNs-pDNA treatment, despite the absence of oxidative stress induction, caused cell cycle arrest in G0/G1 phase and as consequence led to premature senescence, which turned out to be both, p21-dependent and p21-independent. Also, observed DNMT2 upregulation may suggest the activation of different pathways protecting from the resulting CNs-mediated stress. In conclusion, treatment of different cell lines with CNs-pDNA showed that their biocompatibility was limited and effects were cell type-dependent.
Yazar Ek Girişi:
Tek Biçim Eser Adı:
Thesis (Master)--İzmir Institute of Technology: Biotechnology and Bioengineering.

İzmir Institute of Technology: Biotechnology and Bioengineering--Thesis (Master).
Elektronik Erişim:
Access to Electronic Versiyon.
Ayırtma: Copies: