Basic Laboratory Procedures in Clinical Bacteriology. için kapak resmi
Basic Laboratory Procedures in Clinical Bacteriology.
Başlık:
Basic Laboratory Procedures in Clinical Bacteriology.
Yazar:
Vandepitte, Jozef.
ISBN:
9789240680111
Yazar Ek Girişi:
Basım Bilgisi:
2nd ed.
Fiziksel Tanımlama:
1 online resource (177 pages)
İçerik:
Contents -- Preface -- Introduction -- Quality assurance in bacteriology -- Introduction -- Definitions -- Internal quality control -- External quality assessment -- PART I: Bacteriological investigations -- Blood -- Introduction -- When and where bacteraemia may occur -- Blood collection -- Blood-culture media -- Processing of blood cultures -- Cerebrospinal fluid -- Introduction -- Collection and transportation of specimens -- Macroscopic inspection -- Microscopic examination -- Preliminary identification -- Susceptibility testing -- Urine -- Introduction -- Specimen collection -- Culture and interpretation -- Interpretation of quantitative urine culture results -- Identification -- Susceptibility tests -- Stool -- Introduction -- Etiological agents and clinical features -- Appropriate use of laboratory resources -- Collection and transport of stool specimens -- Visual examination of stool specimens -- Enrichment and inoculation of stool specimens -- Media for enteric pathogens -- Primary isolation -- Preliminary identification of isolates -- Final microbiological identification -- Serological identification -- Upper respiratory tract infections -- Introduction -- Normal flora of the pharynx -- Bacterial agents of pharyngitis -- Collection and dispatch of specimens -- Direct microscopy -- Culture and identification -- Susceptibility testing -- Lower respiratory tract infections -- Introduction -- The most common infections -- Collection of sputum specimens -- Processing of sputum in the laboratory (for non-tuberculous infections) -- Culture for Mycobacterium tuberculosis -- Interpretation of cultures for M. tuberculosis -- General note on safety -- Sexually transmitted diseases -- Introduction -- Urethritis in men -- Genital specimens from women -- Specimens from genital ulcers -- Purulent exudates, wounds and abscesses -- Introduction.

Commonly encountered clinical conditions and the most frequent etiological agents -- Collection and transportation of specimens -- Macroscopic evaluation -- Microscopic examination -- Culture -- Identification -- Susceptibility testing -- Anaerobic bacteriology -- Introduction -- Description of bacteria in relation to oxygen requirement -- Bacteriology -- Antimicrobial susceptibility testing -- Introduction -- General principles of antimicrobial susceptibility testing -- Clinical definition of terms "resistant" and "susceptible": the three category system -- Indications for routine susceptibility tests -- Choice of drugs for routine susceptibility tests in the clinical laboratory -- The modified Kirby-Bauer method -- Direct versus indirect susceptibility tests -- Technical factors influencing the size of the zone in the disc-diffusion method -- Quality control -- Serological tests -- Introduction -- Quality control measures -- Serological reactions -- Serological tests for syphilis -- Febrile agglutinins tests -- Antistreptolysin O test -- Bacterial antigen tests -- PART II: Essential media and reagents -- Introduction -- Pathogens, media and diagnostic reagents -- Blood -- Cerebrospinal fluid -- Urine -- Stool -- Upper respiratory tract -- Lower respiratory tract -- Urogenital specimens for exclusion of sexually transmitted diseases -- Pus and exudates -- List of recommended media and diagnostic reagents for the intermediate microbiological laboratory -- Selected further reading -- Index -- A -- B -- C -- D -- E -- F -- G -- H -- I -- J -- K -- L -- M -- N -- O -- P -- Q -- R -- S -- T -- U -- V -- W -- X -- Y -- Z.
Özet:
This manual is a practical guide, for use by laboratory workers in health centres and district hospitals, to the procedures to be followed in obtaining specimens, isolating and identifying bacteria, and assessing their resistance to antibiotics. It covers bacteriological investigation of blood, cerebrospinal fluid, urine, stool, sputum, pharyngeal and genital specimens, and purulent exudates. Particular attention is given to the need for quality control of all laboratory procedures. A list of media and reagents needed for the isolation and identification of the most common bacterial pathogens is included, together with an indication of their relative importance for the intermediary laboratory. This list is intended for adaptation to local circumstances. This second edition has been updated in many areas, including a greatly enhanced section on stool specimens and a new section on serological tests.
Notlar:
Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, 2017. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries.
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