CONTENTS -- Foreword Owen M. Rennert -- Chapter 1 The roles of mouse Y chromosome genes in spermatogenesis Paul S. Burgoyne and Michael J. Mitchell -- Introduction -- The Structure and Gene Content of the mouse Y Chromosome -- The Yd1 Deletion Causes an Increase in Sperm Defects and Rbmy Deficiency -- NPYq Deletions Affect Sperm Development, with Larger Deletions Causing Sterility -- Evidence That an NPYq Gene(s) Represses X Gene Expression in Spermatids -- The Consequences of Sex Chromosome Univalence at the First Meiotic Metaphase -- Eif2s3y from the Sxrb Interval on Yp Has a Role in Spermatogonial Proliferation -- Evidence for Further Spermatogenic Functions on Yp Mapping to Sxrb -- Secondary Changes in the Testicular Phenotype in Mice with Y Deficiencies -- Acknowledgments -- References -- Note Added in Proof -- Chapter 2 Male meiotic sex chromosome inactivation and meiotic silencing James M. A. Turner and Paul S. Burgoyne -- Meiotic Sex Chromosome Inactivation (MSCI) -- Molecular Genetics of MSCI -- MSCI as a Special Example of a General Meiotic Silencing Mechanism -- The Role of MSCI Reconsidered -- Readdressing the Ontogeny of MSCI -- Concluding Remarks -- Acknowledgments -- References -- Chapter 3 Insights into SRY action from sex reversal mutations Melissa I. Stahle, Pascal Bernard, and Vincent R. Harley -- Sex Determination -- Other Human Sex-Determining Genes -- SOX9 -- SF-1 -- DAX-1 -- DHH -- WT1 -- WNT4 -- Additional loci -- Clinical Mutations of SRY -- Function Consequences of SRYMutations -- DNA binding and bending -- Nuclear import -- Protein Interaction with KRAB-O -- KRAB-O -- Domain structure -- Mechanisms of function -- Model: the role of the SRY-KRAB-O complex in sex determination -- Conclusions -- Acknowledgments -- References -- Chapter 4 The TSPY gene family Yun-Fai Chris Lau, Tatsuo Kido, and Yunmin Li.

The Gonadoblastoma Locus and the TSPY Gene on the Human Y Chromosome -- Properties of the Testis-Specific Protein Y-Encoded (TSPY) Gene -- TSPX is an X-Located TSPY Homolog with Contrasting Properties -- Organization of TSPY-Like Genes and Proteins -- Expression of TSPY and TSPY-Like Genes in the Mouse -- Acknowledgments -- References -- Chapter 5 Structure and function of AZFa locus in human spermatogenesis Peter H. Vogt, Hans-Juergen Ditton, Christine Kamp, and Jutta Zimmer -- Introduction -- Human Spermatogoniogenesis During Embryogenesis -- Human Spermatogoniogenesis After Birth -- Genomic Structure of the AZFa Locus in Proximal Yq11 -- Structure and Function of the USP9Y Gene and Its Functional Homologs -- Structure and Function of the DBY Gene and Its Functional Homologs -- Structure and Function of the UTY Gene and Its Functional Homologs -- AZFa Genes and the Gonadoblastoma Y (GBY) Locus in Yq11 -- Outlook -- Acknowledgments -- References -- Chapter 6 RBMY and DAZ in spermatogenesis Pauline Yen -- Introduction -- The RBMY Gene Family -- Gene and protein structure -- Expression and biological functions -- The DAZ Gene Family -- Gene and protein structure -- Deletions and duplications -- The DAZ gene family and gametogenesis -- Biological functions -- Conclusion -- References -- Chapter 7 Neurotrophic factors in the development of the postnatal male germ line Marie-Claude Hofmann and Laura Braydich-Stolle -- Introduction -- Neurotrophic Factors -- Neurotrophins and their receptors -- Nerve growth factor (NGF) and its receptors -- Brain-derived neurotrophic factor (BDNF) and its receptors -- Neurotrophin-3 (NT-3) and its receptors -- Neurotrophin-4/5 (NT-4/5) and its receptors -- Targeted gene disruption and signaling pathways -- Neuropoietic cytokines -- Leukemia inhibitory factor (LIF) -- Ciliary neurotrophic factor (CNTF).

Oncostatin M (OSM) -- Interleukin-6 (IL-6) and interleukin-11 (IL-11) -- Signal transduction by neuropoietic cytokines and gene targeting -- GDNF family of neurotrophic factors -- GDNF-knockout mice and transgenic studies -- Ret receptor tyrosine kinase and signal transduction -- Cooperation of GDNF with other growth factors and signaling pathways -- Summary and Conclusions -- Acknowledgments -- References -- Chapter 8 Dickkopf-like 1-a protein unique to mammals that is associated both with formation of trophoblast stem cells and with spermatogenesis . ..... ....... ....... ..... Matthew J. Kohn, Kotaro J. Kaneko, Rieko Yagi, Eveline S. Litscher, Paul M. Wa -- The Role of Dickkopf Genes in the Regulation of Animal Development -- Expression of Dickkopf Genes During Mammalian Development -- Dkkl1 -- Differential Expression of Dkkl1 and Tead2 Genes -- Regulation of Dkkl1 and Tead2 Gene Expression -- Dkkl1 and Spermatogenesis -- What Is the Function of Dkkl1? -- References -- Chapter 9 Antisense transcription in developing male germ cells Wai-Yee Chan, Shao-Ming Wu, Lisa Ruszczyk, Tin-Lap Lee, and Owen M. Rennert -- Introduction -- Potential Function of Antisense Transcripts -- Antisense Transcription in Germ Cells -- Studies of the Expression of Antisense Transcripts -- Stage-Specific Expression of Antisense Transcripts -- Structural Complexity of Antisense Transcripts Identified in Germ Cells -- Group 1 antisense transcripts -- Group 2 antisense transcripts -- Group 3 antisense transcripts -- Conclusion -- Acknowledgments -- References -- Chapter 10 The spermatogonial stem cell model Martin Dym and Zuping He -- Introduction -- Isolation and Characterization of Spermatogonial Stem Cells -- Procollagen I in the Spermatogonial Cells -- Synthetic Sex Cells -- Novel Methods of Isolating Testis Stem Cells -- Summary -- Acknowledgments -- References.

Chapter 11 Transplantation of germ cells and testis tissue Ina Dobrinski -- Introduction -- Technical Aspects of Germ Cell Transplantation -- Characterization of Spermatogonial Stem Cells -- Characterization of the Stem Cell Niche -- Germ Cell Culture -- Characterization of Spermatogenic Defects -- Germ Cell Transplantation Between Different Species -- Germ Cell Transplantation in Higher Mammalian Species -- Xenografting of Testis Tissue -- Applications for Transplantation of Germ Cells and Testis Tissue -- Conclusions -- Acknowledgments -- References -- Chapter 12 Orthodox and unorthodox ways to initiate fertilization and development in mammals Ryuzo Yanagimachi -- Introduction -- Preparation of Germ Cells for Fertilization -- Assisted Fertilization -- Cloning -- References -- Chapter 13 Pathogenesis of testicular germ cell tumors Leendert H. J. Looijenga -- Introduction -- Classification of Human GCTs: Limitations and Possibilities -- Normal and Abnormal Development in Human GCTs -- Genomic Imprinting and Cell of Origin of Human GCTs -- Risk Factors for Human GCTs -- Genomic Anomalies and Candidate Genes -- Type I GCTs -- Type II GCTs -- Type III GCTs -- Omnipotency of Type II GCTs and OCT3/4-POU5F1 as a Diagnostic Marker -- The Y Chromosome and Human GCTs -- OCT3/4-POU5F1 and TSPY Double-Positive Cells at Risk for Malignant Transformation? -- Animal Models for Human GCTs -- Concluding Remarks -- Acknowledgments -- References -- Chapter 14 Origin of testicular germ cell neoplasia: the role of sex chromosomes Ewa Rajpert-De Meyts, Anne Marie Ottesen, Christina Hoei-Hansen, Si Brask Sonne, Henrik Leffers, and Niels E. Skakkebæk -- Introduction -- Testis Carcinoma In Situ and Gonadoblastoma: Phenotypic Features -- Aneuploidy and Structural Abnormalities of the Y Chromosome -- Aneuploidy and Possible Structural Abnormalities of the X Chromosome.

The Current Understanding of the Origin of Germ Cell Neoplasia -- Acknowledgments -- References -- Author Index -- Index.